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Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes
The potential genotoxicity (nuclear anomalies, damage to single‐strand DNA) and pinocytic adherence activity of two (glyphosate‐based and paraquat‐based) commercial herbicides to earthworm coelomocytes (immune cells in the coelomic cavity) were assessed. Coelomocytes were extracted from earthworms (Pheretima peguana) exposed to concentrations P. peguana. There were significant (P < 0.05) differences from the control group in total coelomocyte micronuclei, binuclei, and trinuclei frequencies of earthworms exposed to glyphosate at 25 × 10–1 (10–3 LC50) and paraquat at 39 × 10–5 (10–4 LC50) μg cm–2 filter paper. In earthworms exposed to glyphosate, no differences in tail DNA%, tail length, and tail moment of coelomocytes were detected. In contrast, for paraquat at 10–1 LC50 concentration, there were significant (P < 0.05) differences between tail DNA % and tail length, and at LC50 concentration, tail moment was also significantly different when compared with controls. A decline in pinocytic adherence activity in coelomocytes occurred on exposure to glyphosate or paraquat at 10–3 LC50 concentration. This study showed that, at concentrations well below field application rates, paraquat induces both clastogenic and aneugenic effects on earthworm coelomocytes whereas glyphosate causes only aneugenic effects and therefore does not pose a risk of gene mutation in this earthworm. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 612–620, 2014.
Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes
The potential genotoxicity (nuclear anomalies, damage to single‐strand DNA) and pinocytic adherence activity of two (glyphosate‐based and paraquat‐based) commercial herbicides to earthworm coelomocytes (immune cells in the coelomic cavity) were assessed. Coelomocytes were extracted from earthworms (Pheretima peguana) exposed to concentrations P. peguana. There were significant (P < 0.05) differences from the control group in total coelomocyte micronuclei, binuclei, and trinuclei frequencies of earthworms exposed to glyphosate at 25 × 10–1 (10–3 LC50) and paraquat at 39 × 10–5 (10–4 LC50) μg cm–2 filter paper. In earthworms exposed to glyphosate, no differences in tail DNA%, tail length, and tail moment of coelomocytes were detected. In contrast, for paraquat at 10–1 LC50 concentration, there were significant (P < 0.05) differences between tail DNA % and tail length, and at LC50 concentration, tail moment was also significantly different when compared with controls. A decline in pinocytic adherence activity in coelomocytes occurred on exposure to glyphosate or paraquat at 10–3 LC50 concentration. This study showed that, at concentrations well below field application rates, paraquat induces both clastogenic and aneugenic effects on earthworm coelomocytes whereas glyphosate causes only aneugenic effects and therefore does not pose a risk of gene mutation in this earthworm. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 612–620, 2014.
Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes
Muangphra, Ptumporn (author) / Kwankua, Wimon (author) / Gooneratne, Ravi (author)
Environmental Toxicology ; 29 ; 612-620
2014-06-01
9 pages
Article (Journal)
Electronic Resource
English
Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes
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