A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Antrodia camphorata attenuates cigarette smoke‐induced ROS production, DNA damage, apoptosis, and inflammation in vascular smooth muscle cells, and atherosclerosis in ApoE‐deficient mice
Cigarette smoke exposure activates several cellular mechanisms predisposing to atherosclerosis, including oxidative stress, dyslipidemia, and vascular inflammation. Antrodia camphorata, a renowned medicinal mushroom in Taiwan, has been investigated for its antioxidant, anti‐inflammatory, and antiatherosclerotic properties in cigarette smoke extracts (CSE)‐treated vascular smooth muscle cells (SMCs), and ApoE‐deficient mice. Fermented culture broth of Antrodia camphorata (AC, 200‐800 µg/mL) possesses effective antioxidant activity against CSE‐induced ROS production. Treatment of SMCs (A7r5) with AC (30‐120 µg/mL) remarkably ameliorated CSE‐induced morphological aberrations and cell death. Suppressed ROS levels by AC corroborate with substantial inhibition of CSE‐induced DNA damage in AC‐treated A7r5 cells. We found CSE‐induced apoptosis through increased Bax/Bcl‐2 ratio, was substantially inhibited by AC in A7r5 cells. Notably, upregulated SOD and catalase expressions in AC‐treated A7r5 cells perhaps contributed to eradicate the CSE‐induced ROS generation, and prevents DNA damage and apoptosis. Besides, AC suppressed AP‐1 activity by inhibiting the c‐Fos/c‐Jun expressions, and NF‐κB activation through inhibition of I‐κBα degradation against CSE‐stimulation. This anti‐inflammatory property of AC was accompanied by suppressed CSE‐induced VEGF, PDGF, and EGR‐1 overexpressions in A7r5 cells. Furthermore, AC protects lung fibroblast (MRC‐5) cells from CSE‐induced cell death. In vivo data showed that AC oral administration (0.6 mg/d/8‐wk) prevents CSE‐accelerated atherosclerosis in ApoE‐deficient mice. This antiatherosclerotic property was associated with increased serum total antioxidant status, and decreased total cholesterol and triacylglycerol levels. Thus, Antrodia camphorata may be useful for prevention of CSE‐induced oxidative stress and diseases. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2070–2084, 2017.
Antrodia camphorata attenuates cigarette smoke‐induced ROS production, DNA damage, apoptosis, and inflammation in vascular smooth muscle cells, and atherosclerosis in ApoE‐deficient mice
Cigarette smoke exposure activates several cellular mechanisms predisposing to atherosclerosis, including oxidative stress, dyslipidemia, and vascular inflammation. Antrodia camphorata, a renowned medicinal mushroom in Taiwan, has been investigated for its antioxidant, anti‐inflammatory, and antiatherosclerotic properties in cigarette smoke extracts (CSE)‐treated vascular smooth muscle cells (SMCs), and ApoE‐deficient mice. Fermented culture broth of Antrodia camphorata (AC, 200‐800 µg/mL) possesses effective antioxidant activity against CSE‐induced ROS production. Treatment of SMCs (A7r5) with AC (30‐120 µg/mL) remarkably ameliorated CSE‐induced morphological aberrations and cell death. Suppressed ROS levels by AC corroborate with substantial inhibition of CSE‐induced DNA damage in AC‐treated A7r5 cells. We found CSE‐induced apoptosis through increased Bax/Bcl‐2 ratio, was substantially inhibited by AC in A7r5 cells. Notably, upregulated SOD and catalase expressions in AC‐treated A7r5 cells perhaps contributed to eradicate the CSE‐induced ROS generation, and prevents DNA damage and apoptosis. Besides, AC suppressed AP‐1 activity by inhibiting the c‐Fos/c‐Jun expressions, and NF‐κB activation through inhibition of I‐κBα degradation against CSE‐stimulation. This anti‐inflammatory property of AC was accompanied by suppressed CSE‐induced VEGF, PDGF, and EGR‐1 overexpressions in A7r5 cells. Furthermore, AC protects lung fibroblast (MRC‐5) cells from CSE‐induced cell death. In vivo data showed that AC oral administration (0.6 mg/d/8‐wk) prevents CSE‐accelerated atherosclerosis in ApoE‐deficient mice. This antiatherosclerotic property was associated with increased serum total antioxidant status, and decreased total cholesterol and triacylglycerol levels. Thus, Antrodia camphorata may be useful for prevention of CSE‐induced oxidative stress and diseases. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2070–2084, 2017.
Antrodia camphorata attenuates cigarette smoke‐induced ROS production, DNA damage, apoptosis, and inflammation in vascular smooth muscle cells, and atherosclerosis in ApoE‐deficient mice
Yang, Hsin‐Ling (author) / Korivi, Mallikarjuna (author) / Chen, Cheng‐Hsien (author) / Peng, Wei‐Jung (author) / Chen, Chee‐Shan (author) / Li, Mei‐Ling (author) / Hsu, Li‐Sung (author) / Liao, Jiunn‐Wang (author) / Hseu, You‐Cheng (author)
Environmental Toxicology ; 32 ; 2070-2084
2017-08-01
15 pages
Article (Journal)
Electronic Resource
English
Humic acid induces G1 phase arrest and apoptosis in cultured vascular smooth muscle cells
| Online Contents | 2009
Cross-Talk Between PCSK9 and Damaged mtDNA in Vascular Smooth Muscle Cells: Role in Apoptosis
British Library Online Contents | 2016