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Unveiling the Coupling Mechanism between Central Carbon and Nitrogen Metabolism of Pseudomonas stutzeri: Polyhydroxyalkanoate-Enhanced Electron Supply for Denitrification
https://orcid.org/0009-0008-1826-9434
https://orcid.org/0000-0002-9153-3093
https://orcid.org/0000-0001-9685-5510
High nitrogen levels in drinking water sources pose a public health threat and have drawn significant attention. In situ high-efficiency ecological restoration technology using polyhydroxyalkanoates (PHAs) can effectively enrich target microorganisms and enhance denitrification in water bodies. However, the mechanisms behind PHA-driven carbon metabolism, nitrogen transformation, and electron transfer in denitrification remain unclear. This study investigated Pseudomonas stutzeri using sodium acetate (NaAc) or sodium 3-hydroxybutyrate as the sole carbon source. The PHA group achieved superior nitrogen removal (171.44 mg/L N, 13.39 mg N/mg DNA) compared to the NaAc group (83.83 mg/L N, 7.98 mg N/mg DNA), driven by elevated NADH levels and an increased NADH/NAD+ ratio, facilitating electron transfer to denitrifying enzymes (i.e., Nap, Nir). The metabolic flux shifted, with the tricarboxylic acid (TCA) cycle decreasing 7.08-fold and the pentose phosphate pathway (PPP) increasing 2.53-fold, optimizing reducing equivalent production for nitrogen assimilation and denitrification. A potential mechanism for PHA-enhanced denitrification and new insights into how carbon substrates regulate microbial functions in denitrification were then proposed.
Polyhydroxyalkanoate’s metabolic preference for the pentose phosphate pathway rather than the TCA cycle reconstructed the intracellular redox balance, enhancing electron supply during the denitrification process.
Unveiling the Coupling Mechanism between Central Carbon and Nitrogen Metabolism of Pseudomonas stutzeri: Polyhydroxyalkanoate-Enhanced Electron Supply for Denitrification
https://orcid.org/0009-0008-1826-9434
https://orcid.org/0000-0002-9153-3093
https://orcid.org/0000-0001-9685-5510
High nitrogen levels in drinking water sources pose a public health threat and have drawn significant attention. In situ high-efficiency ecological restoration technology using polyhydroxyalkanoates (PHAs) can effectively enrich target microorganisms and enhance denitrification in water bodies. However, the mechanisms behind PHA-driven carbon metabolism, nitrogen transformation, and electron transfer in denitrification remain unclear. This study investigated Pseudomonas stutzeri using sodium acetate (NaAc) or sodium 3-hydroxybutyrate as the sole carbon source. The PHA group achieved superior nitrogen removal (171.44 mg/L N, 13.39 mg N/mg DNA) compared to the NaAc group (83.83 mg/L N, 7.98 mg N/mg DNA), driven by elevated NADH levels and an increased NADH/NAD+ ratio, facilitating electron transfer to denitrifying enzymes (i.e., Nap, Nir). The metabolic flux shifted, with the tricarboxylic acid (TCA) cycle decreasing 7.08-fold and the pentose phosphate pathway (PPP) increasing 2.53-fold, optimizing reducing equivalent production for nitrogen assimilation and denitrification. A potential mechanism for PHA-enhanced denitrification and new insights into how carbon substrates regulate microbial functions in denitrification were then proposed.
Polyhydroxyalkanoate’s metabolic preference for the pentose phosphate pathway rather than the TCA cycle reconstructed the intracellular redox balance, enhancing electron supply during the denitrification process.
Unveiling the Coupling Mechanism between Central Carbon and Nitrogen Metabolism of Pseudomonas stutzeri: Polyhydroxyalkanoate-Enhanced Electron Supply for Denitrification
https://orcid.org/0009-0008-1826-9434
https://orcid.org/0000-0002-9153-3093
https://orcid.org/0000-0001-9685-5510
High nitrogen levels in drinking water sources pose a public health threat and have drawn significant attention. In situ high-efficiency ecological restoration technology using polyhydroxyalkanoates (PHAs) can effectively enrich target microorganisms and enhance denitrification in water bodies. However, the mechanisms behind PHA-driven carbon metabolism, nitrogen transformation, and electron transfer in denitrification remain unclear. This study investigated Pseudomonas stutzeri using sodium acetate (NaAc) or sodium 3-hydroxybutyrate as the sole carbon source. The PHA group achieved superior nitrogen removal (171.44 mg/L N, 13.39 mg N/mg DNA) compared to the NaAc group (83.83 mg/L N, 7.98 mg N/mg DNA), driven by elevated NADH levels and an increased NADH/NAD+ ratio, facilitating electron transfer to denitrifying enzymes (i.e., Nap, Nir). The metabolic flux shifted, with the tricarboxylic acid (TCA) cycle decreasing 7.08-fold and the pentose phosphate pathway (PPP) increasing 2.53-fold, optimizing reducing equivalent production for nitrogen assimilation and denitrification. A potential mechanism for PHA-enhanced denitrification and new insights into how carbon substrates regulate microbial functions in denitrification were then proposed.
Polyhydroxyalkanoate’s metabolic preference for the pentose phosphate pathway rather than the TCA cycle reconstructed the intracellular redox balance, enhancing electron supply during the denitrification process.
Unveiling the Coupling Mechanism between Central Carbon and Nitrogen Metabolism of Pseudomonas stutzeri: Polyhydroxyalkanoate-Enhanced Electron Supply for Denitrification
Zhou, Jieying (Autor:in) / Li, Tingting (Autor:in) / Hu, Dian (Autor:in) / Wu, Boran (Autor:in) / Chai, Xiaoli (Autor:in)
ACS ES&T Water ; 5 ; 230-241
10.01.2025
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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