Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Complementary effect of thermotolerant yeast and cold active cellulase on simultaneous saccharification and fermentation for bioethanol production from rice straw
The present investigation aimed towards the optimization of bioethanol production from alkali pretreated rice straw using thermotolerant Saccharomyces cerevisiae JRC6 strain which was capable of fermenting glucose at 40 °C with a fermentation efficiency of 91.14%. Four process variables, namely, substrate loading (4.0%–7.0% w/v), enzyme loading (10–30 FPU/g dry rice straw), pH (4.0–6.0), and inoculum rate (7.0%–15.0% v/v), were evaluated by response surface methodology using Box Behnken Design to develop a simultaneous saccharification and fermentation process for bioethanol production using thermotolerant yeast and cold active cellulase cocktail produced by Aspergillus niger SH3. Among these variables, pH was the most significant factor followed by substrate loading. The optimum outcomes of the design were as follows: substrate loading (6.71% w/v), enzyme loading (25.71 FPU/g dry rice straw), pH (4.15), and inoculum rate (13.2% v/v). The validation experiment with the optimized parameters resulted in a better ethanol concentration of 12.95±0.39 g/L within 24 h at 40 °C. The results obtained indicated that the process may further be scaled up as a single pot process with substantial energy saving because the saccharification temperature was 10 °C lower as compared to commercial cellulases, and one step has been reduced due to the combined action of the enzyme and yeast.
Complementary effect of thermotolerant yeast and cold active cellulase on simultaneous saccharification and fermentation for bioethanol production from rice straw
The present investigation aimed towards the optimization of bioethanol production from alkali pretreated rice straw using thermotolerant Saccharomyces cerevisiae JRC6 strain which was capable of fermenting glucose at 40 °C with a fermentation efficiency of 91.14%. Four process variables, namely, substrate loading (4.0%–7.0% w/v), enzyme loading (10–30 FPU/g dry rice straw), pH (4.0–6.0), and inoculum rate (7.0%–15.0% v/v), were evaluated by response surface methodology using Box Behnken Design to develop a simultaneous saccharification and fermentation process for bioethanol production using thermotolerant yeast and cold active cellulase cocktail produced by Aspergillus niger SH3. Among these variables, pH was the most significant factor followed by substrate loading. The optimum outcomes of the design were as follows: substrate loading (6.71% w/v), enzyme loading (25.71 FPU/g dry rice straw), pH (4.15), and inoculum rate (13.2% v/v). The validation experiment with the optimized parameters resulted in a better ethanol concentration of 12.95±0.39 g/L within 24 h at 40 °C. The results obtained indicated that the process may further be scaled up as a single pot process with substantial energy saving because the saccharification temperature was 10 °C lower as compared to commercial cellulases, and one step has been reduced due to the combined action of the enzyme and yeast.
Complementary effect of thermotolerant yeast and cold active cellulase on simultaneous saccharification and fermentation for bioethanol production from rice straw
Choudhary, Jairam (Autor:in) / Singh, Surender (Autor:in) / Sharma, Anamika (Autor:in) / Tiwari, Rameshwar (Autor:in) / Nain, Lata (Autor:in)
01.07.2018
11 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Simultaneous Saccharification and Fermentation Using Environmental-adapted Yeast by Preculture
| British Library Online Contents | 2016