Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Azole-Resistant Aspergillus fumigatus Harboring the TR34/L98H Mutation: First Report in Portugal in Environmental Samples
Introduction: The frequency in detection of azole-resistant Aspergillus fumigatus isolates has increased since 2010. In Portugal, the section Fumigati is one of the most frequent, and resistant strains to have been found in clinical and environmental contexts. Although several cryptic species within the Fumigati section show intrinsic resistance to azoles, one factor driving (acquired) resistance is selective pressure deriving from the extensive use of azoles. This is particularly problematic in occupational environments where high fungal loads are expected, and where there is an increased risk of human exposure and infection, with impact on treatment success and disease outcome. The mechanisms of resistance are diverse, but mainly associated with mutations in the cyp51A gene. Despite TR34/L98H being the most frequent mutation described, it has only been detected in clinical specimens in Portugal. Methods: We analyzed 99 A. fumigatus isolates from indoor environments (healthcare facilities, spas, one dairy and one waste sorting unit) collected from January 2018 to February 2019 in different regions of Portugal. Isolates were screened for resistance to itraconazole, voriconazole and posaconazole by culture, and resistance was confirmed by broth microdilution. Sequencing of the cyp51A gene and its promoter was performed to detect mutations associated with resistance. Results: Overall, 8.1% of isolates were able to grow in the presence of at least one azole, and 3% (isolated from the air in a dairy and from filtering respiratory protective devices in a waste sorting industry) were pan-azole-resistant, bearing the TR34/L98H mutation. Conclusion: For the first time in Portugal, we report environmental isolates bearing the TR34/L98H mutation, isolated from occupational environments. Environmental surveillance of the emergence of azole-resistant A. fumigatus sensu stricto strains is needed, to ensure proper and timely implementation of control policies that may have a positive impact on public and occupational health. ; This work was supported by: FCT—Fundação para Ciência e Tecnologia, for funding the project “EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings” (02/SAICT/2016—Project no. 23222); Instituto Politécnico de Lisboa, Lisboa, Portugal, for funding the project “Waste Workers’ Exposure to Bio-burden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL); and by Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa, Portugal, for funding the project “Programa de Vigilância de Aspergillus”. ; info:eu-repo/semantics/publishedVersion
Azole-Resistant Aspergillus fumigatus Harboring the TR34/L98H Mutation: First Report in Portugal in Environmental Samples
Introduction: The frequency in detection of azole-resistant Aspergillus fumigatus isolates has increased since 2010. In Portugal, the section Fumigati is one of the most frequent, and resistant strains to have been found in clinical and environmental contexts. Although several cryptic species within the Fumigati section show intrinsic resistance to azoles, one factor driving (acquired) resistance is selective pressure deriving from the extensive use of azoles. This is particularly problematic in occupational environments where high fungal loads are expected, and where there is an increased risk of human exposure and infection, with impact on treatment success and disease outcome. The mechanisms of resistance are diverse, but mainly associated with mutations in the cyp51A gene. Despite TR34/L98H being the most frequent mutation described, it has only been detected in clinical specimens in Portugal. Methods: We analyzed 99 A. fumigatus isolates from indoor environments (healthcare facilities, spas, one dairy and one waste sorting unit) collected from January 2018 to February 2019 in different regions of Portugal. Isolates were screened for resistance to itraconazole, voriconazole and posaconazole by culture, and resistance was confirmed by broth microdilution. Sequencing of the cyp51A gene and its promoter was performed to detect mutations associated with resistance. Results: Overall, 8.1% of isolates were able to grow in the presence of at least one azole, and 3% (isolated from the air in a dairy and from filtering respiratory protective devices in a waste sorting industry) were pan-azole-resistant, bearing the TR34/L98H mutation. Conclusion: For the first time in Portugal, we report environmental isolates bearing the TR34/L98H mutation, isolated from occupational environments. Environmental surveillance of the emergence of azole-resistant A. fumigatus sensu stricto strains is needed, to ensure proper and timely implementation of control policies that may have a positive impact on public and occupational health. ; This work was supported by: FCT—Fundação para Ciência e Tecnologia, for funding the project “EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings” (02/SAICT/2016—Project no. 23222); Instituto Politécnico de Lisboa, Lisboa, Portugal, for funding the project “Waste Workers’ Exposure to Bio-burden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL); and by Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa, Portugal, for funding the project “Programa de Vigilância de Aspergillus”. ; info:eu-repo/semantics/publishedVersion
Azole-Resistant Aspergillus fumigatus Harboring the TR34/L98H Mutation: First Report in Portugal in Environmental Samples
Gonçalves, Paulo (Autor:in) / Melo, Aryse (Autor:in) / Dias, Marta (Autor:in) / Almeida, Beatriz (Autor:in) / Caetano, Liliana Aranha (Autor:in) / Veríssimo, Cristina (Autor:in) / Viegas, Carla (Autor:in) / Sabino, Raquel (Autor:in)
09.03.2021
doi:10.3390/microorganisms9010057
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
DDC:
690
Surface regularity of Industrial floors - a review of TR34 2003
| British Library Online Contents | 2005
INDUSTRIAL FLOORS - Surface regularity of industrial floors -- A review of TR34 2003
| Online Contents | 2005