Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation: first report in Portugal in environmental sample
Project EXPOsE. Project nº 23222 (02/SAICT/2016). ; Project Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL. ; Introduction: The frequency in the detection of azole-resistant Aspergillus fumigatus isolates has increased since 2010. In Portugal, the section Fumigati is one of the most frequent, and resistant strains to have been found in clinical and environmental contexts. Although several cryptic species within the Fumigati section show intrinsic resistance to azoles, one-factor driving (acquired) resistance is selective pressure deriving from the extensive use of azoles. This is particularly problematic in occupational environments where high fungal loads are expected, and where there is an increased risk of human exposure and infection, with an impact on treatment success and disease outcome. The mechanisms of resistance are diverse but mainly associated with mutations in the cyp51A gene. Despite TR34/L98H being the most frequent mutation described, it has only been detected in clinical specimens in Portugal. Methods: We analyzed 99 A. fumigatus isolates from indoor environments (healthcare facilities, spas, one dairy, and one waste sorting unit) collected from January 2018 to February 2019 in different regions of Portugal. Isolates were screened for resistance to itraconazole, voriconazole, and posaconazole by culture, and resistance was confirmed by broth microdilution. Sequencing of the cyp51A gene and its promoter was performed to detect mutations associated with resistance. Results: Overall, 8.1% of isolates were able to grow in the presence of at least one azole, and 3% (isolated from the air in a dairy and from filtering respiratory protective devices in a waste sorting industry) were pan-azole-resistant, bearing the TR34/L98H mutation. Conclusion: For the first time in Portugal, we report environmental isolates bearing the TR34/L98H mutation, isolated from occupational environments. Environmental surveillance of the emergence of azole-resistant A. fumigatus sensu stricto strains is needed, to ensure ...
Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation: first report in Portugal in environmental sample
Project EXPOsE. Project nº 23222 (02/SAICT/2016). ; Project Waste FRPD. Project IPL/2018/WasteFRPD_ESTeSL. ; Introduction: The frequency in the detection of azole-resistant Aspergillus fumigatus isolates has increased since 2010. In Portugal, the section Fumigati is one of the most frequent, and resistant strains to have been found in clinical and environmental contexts. Although several cryptic species within the Fumigati section show intrinsic resistance to azoles, one-factor driving (acquired) resistance is selective pressure deriving from the extensive use of azoles. This is particularly problematic in occupational environments where high fungal loads are expected, and where there is an increased risk of human exposure and infection, with an impact on treatment success and disease outcome. The mechanisms of resistance are diverse but mainly associated with mutations in the cyp51A gene. Despite TR34/L98H being the most frequent mutation described, it has only been detected in clinical specimens in Portugal. Methods: We analyzed 99 A. fumigatus isolates from indoor environments (healthcare facilities, spas, one dairy, and one waste sorting unit) collected from January 2018 to February 2019 in different regions of Portugal. Isolates were screened for resistance to itraconazole, voriconazole, and posaconazole by culture, and resistance was confirmed by broth microdilution. Sequencing of the cyp51A gene and its promoter was performed to detect mutations associated with resistance. Results: Overall, 8.1% of isolates were able to grow in the presence of at least one azole, and 3% (isolated from the air in a dairy and from filtering respiratory protective devices in a waste sorting industry) were pan-azole-resistant, bearing the TR34/L98H mutation. Conclusion: For the first time in Portugal, we report environmental isolates bearing the TR34/L98H mutation, isolated from occupational environments. Environmental surveillance of the emergence of azole-resistant A. fumigatus sensu stricto strains is needed, to ensure ...
Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation: first report in Portugal in environmental sample
Gonçalves, Paulo (Autor:in) / Melo, Aryse (Autor:in) / Dias, Marta (Autor:in) / Almeida, Beatriz (Autor:in) / Caetano, Liliana Aranha (Autor:in) / Veríssimo, Cristina (Autor:in) / Viegas, Carla (Autor:in) / Sabino, Raquel (Autor:in)
01.12.2020
doi:10.3390/microorganisms9010057
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
DDC:
690
Surface regularity of Industrial floors - a review of TR34 2003
| British Library Online Contents | 2005
INDUSTRIAL FLOORS - Surface regularity of industrial floors -- A review of TR34 2003
| Online Contents | 2005