Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Integration of hygienically relevant bacteria in drinking water biofilms grown on domestic plumbing materials ; Einnistung hygienisch relevanter Bakterien in Trinkwasserbiofilme auf Werkstoffen der Trinkwasser-Installation
Biofilms in domestic plumbing systems can represent a reservoir for potentially pathogenic bacteria including Pseudomonas aeruginosa, Legionella pneumophila and coliform bacteria. The selection of materials utilised in domestic plumbing as well as their exposure to disinfectant stress (“ageing”) may affect the extent of biofilm formation, the composition of biofilm populations and the incorporation and persistence of hygienically relevant bacteria in drinking water biofilms. The presence of protozoa may additionally influence the persistence and multiplication of potentially pathogenic bacteria. Drinking water biofilms were grown on coupons of plumbing materials including ethylene-propylene-diene-monomer (EPDM) rubber, silane cross-linked polyethylene (PE-Xb), electron-ray cross-linked PE (PE-Xc) and copper under constant flow-through of cold tap water at ambient temperature (19.0 °C ± 3.1 °C). The materials were tested both untreated and after treatment with sodium hy¬pochlorite (5 ppm, 3 bar, 40 °C, 4 weeks) or chlorine dioxide (5 ppm, 3 bar, 40 °C, 4 weeks) in case of EPDM, PE-X b and c or after exposure to unchlorinated drinking water for ≥ 6 months in case of copper. After 14 days, the biofilms were spiked with Pseudomonas aeruginosa, Legionella pneumophila and Enterobacter nimipressuralis (106 cells/mL each). The test bacteria were environmental isolates from contamination cases in drinking water systems. After static incubation for 24 h, water flow was resumed and continued for four weeks. Total cell count and heterotrophic plate count (HPC) of biofilms were monitored, and the population diversity of was determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). P. aeruginosa, L. pneumophila and E. nimipressuralis were quantified, using standard culture-based methods or culture-independent fluorescence in situ hybridisation (FISH). 14 day-old biofilms grown on untreated EPDM, PE-Xb, PE-X c and copper were analysed for the presence of total protozoa and the amoebal ...
Integration of hygienically relevant bacteria in drinking water biofilms grown on domestic plumbing materials ; Einnistung hygienisch relevanter Bakterien in Trinkwasserbiofilme auf Werkstoffen der Trinkwasser-Installation
Biofilms in domestic plumbing systems can represent a reservoir for potentially pathogenic bacteria including Pseudomonas aeruginosa, Legionella pneumophila and coliform bacteria. The selection of materials utilised in domestic plumbing as well as their exposure to disinfectant stress (“ageing”) may affect the extent of biofilm formation, the composition of biofilm populations and the incorporation and persistence of hygienically relevant bacteria in drinking water biofilms. The presence of protozoa may additionally influence the persistence and multiplication of potentially pathogenic bacteria. Drinking water biofilms were grown on coupons of plumbing materials including ethylene-propylene-diene-monomer (EPDM) rubber, silane cross-linked polyethylene (PE-Xb), electron-ray cross-linked PE (PE-Xc) and copper under constant flow-through of cold tap water at ambient temperature (19.0 °C ± 3.1 °C). The materials were tested both untreated and after treatment with sodium hy¬pochlorite (5 ppm, 3 bar, 40 °C, 4 weeks) or chlorine dioxide (5 ppm, 3 bar, 40 °C, 4 weeks) in case of EPDM, PE-X b and c or after exposure to unchlorinated drinking water for ≥ 6 months in case of copper. After 14 days, the biofilms were spiked with Pseudomonas aeruginosa, Legionella pneumophila and Enterobacter nimipressuralis (106 cells/mL each). The test bacteria were environmental isolates from contamination cases in drinking water systems. After static incubation for 24 h, water flow was resumed and continued for four weeks. Total cell count and heterotrophic plate count (HPC) of biofilms were monitored, and the population diversity of was determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). P. aeruginosa, L. pneumophila and E. nimipressuralis were quantified, using standard culture-based methods or culture-independent fluorescence in situ hybridisation (FISH). 14 day-old biofilms grown on untreated EPDM, PE-Xb, PE-X c and copper were analysed for the presence of total protozoa and the amoebal ...
Integration of hygienically relevant bacteria in drinking water biofilms grown on domestic plumbing materials ; Einnistung hygienisch relevanter Bakterien in Trinkwasserbiofilme auf Werkstoffen der Trinkwasser-Installation
Moritz, Miriam (Autor:in) / Flemming, Hans-Curt
07.06.2011
Hochschulschrift
Elektronische Ressource
Englisch
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