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Transcriptome and clinical analysis of immune cells in viral infectious disease
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of COVID-19. COVID-19 has been declared a pandemic by the World Health Organization (WHO), and is having widely effects on both individual lives and economies around the world. At the early stage of this pandemic, the response of host immunity during infection is unclear. It is not well-understood what initiates and propagates the cytokine storm. Thus, to identify the characteristics of neutrophils, monocytes and lymphocytes involved in the development of COVID-19 pneumonia is an emergency issue. I begin this thesis with an introduction of the function of immune cells with distinct disease severities of COVID-19, as well as a brief introduction of TLR signaling pathway in hepatitis B virus (HBV) infection (chapter 1). This is followed by general introduction of analysis method of clinical and transcriptome data (chapter 2). The main result of this thesis is summarized in three publications about the role of neutrophils, lymphocytes and type one IFN response in the pathology of COVID-19, respectively and anther one publication of HBV particle induced metabolic change in B cells (chapter 3). In the first publication (section 3.1), I analyze a set of laboratory parameters and the corresponding chest CT images of 55 COVID-19 patients during hospitalization. Among these variables, excessive neutrophils were related with CT values in patients with severity of pneumonia. Moreover, The ratio of neutrophils to lymphocytes (NLR) could be a useful factor in assessing the severity of pneumonia. The transcriptome analysis of lung specimens and bronchoalveolar lavage fluid (BALF) from COVID-19 patients indicate that the most up-regulated marker genes from immune cells are neutrophil related. Importantly, many neutrophil activation genes are categorized as NET-associated genes. It is further investigated that these genes interact with T and NK cells via negative regulatory molecules in COVID-19 patients, resulting in an inadequate antiviral response and ...
Transcriptome and clinical analysis of immune cells in viral infectious disease
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of COVID-19. COVID-19 has been declared a pandemic by the World Health Organization (WHO), and is having widely effects on both individual lives and economies around the world. At the early stage of this pandemic, the response of host immunity during infection is unclear. It is not well-understood what initiates and propagates the cytokine storm. Thus, to identify the characteristics of neutrophils, monocytes and lymphocytes involved in the development of COVID-19 pneumonia is an emergency issue. I begin this thesis with an introduction of the function of immune cells with distinct disease severities of COVID-19, as well as a brief introduction of TLR signaling pathway in hepatitis B virus (HBV) infection (chapter 1). This is followed by general introduction of analysis method of clinical and transcriptome data (chapter 2). The main result of this thesis is summarized in three publications about the role of neutrophils, lymphocytes and type one IFN response in the pathology of COVID-19, respectively and anther one publication of HBV particle induced metabolic change in B cells (chapter 3). In the first publication (section 3.1), I analyze a set of laboratory parameters and the corresponding chest CT images of 55 COVID-19 patients during hospitalization. Among these variables, excessive neutrophils were related with CT values in patients with severity of pneumonia. Moreover, The ratio of neutrophils to lymphocytes (NLR) could be a useful factor in assessing the severity of pneumonia. The transcriptome analysis of lung specimens and bronchoalveolar lavage fluid (BALF) from COVID-19 patients indicate that the most up-regulated marker genes from immune cells are neutrophil related. Importantly, many neutrophil activation genes are categorized as NET-associated genes. It is further investigated that these genes interact with T and NK cells via negative regulatory molecules in COVID-19 patients, resulting in an inadequate antiviral response and ...
Transcriptome and clinical analysis of immune cells in viral infectious disease
Wang, Jun (Autor:in) / Hoffmann, Daniel
01.04.2022
Hochschulschrift
Elektronische Ressource
Englisch
Infectious Disease Respiratory Disease Examination Room System
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