Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Immobilization method for improving activity of microorganisms in well cementation cement paste
The invention belongs to the technical field of bioengineering and oil and gas exploitation engineering, and particularly relates to an immobilization method for improving activity of microorganisms in well cementation cement paste, which mainly comprises the following steps: S1, selecting target microorganisms for expanding culture treatment, and taking bacterial liquid after expanding culture for later use; S2, taking a certain amount of porous ceramic particles, flatly laying the porous ceramic particles on a flat plate, uniformly spraying a certain amount of nutrient solution to the surfaces of the porous ceramic particles, and performing standing for 24 hours for later use; S3, preparing target porous ceramic; S4, preparing a microorganism immobilized product; and S5, preparing the microbial well cementation cement paste. The invention discloses the immobilization method for improving the activity of microorganisms in the well cementation cement paste. The microorganisms are immobilized in a porous material in this way, and then the microorganism well cementation cement paste is prepared. The activity of microorganisms in cement paste can be prolonged, porous ceramic particlesand the well cementation cement paste can be solidified into a cementation body with certain impermeability and mechanical properties through the mineralization effect of the microorganisms, and theservice life of the well cementation cement paste can be greatly prolonged.
本发明属于生物工程与油气开采工程技术领域，具体涉及一种提高固井水泥浆中微生物活性的固载方法，主要包括以下步骤：S1、选取目标微生物进行扩培处理，并取扩培后的菌液备用；S2、取定量多孔陶瓷颗粒平铺在平板上，向其表面均匀喷洒定量的营养液后，静置24小时备用；S3、制备目标多孔陶瓷；S4、制备微生物固载产品；S5、制备微生物固井水泥浆。本发明所述的一种提高固井水泥浆中微生物活性的固载方法，将微生物以此种方式固载于多孔材料中再制备微生物固井水泥浆，可以延长微生物在水泥浆中的活性，通过微生物的成矿作用可以将多孔陶瓷微粒与固井水泥浆固结成具有一定抗渗性和力学性能的胶结体，可以大大延长固井水泥环的服役寿命。
Immobilization method for improving activity of microorganisms in well cementation cement paste
The invention belongs to the technical field of bioengineering and oil and gas exploitation engineering, and particularly relates to an immobilization method for improving activity of microorganisms in well cementation cement paste, which mainly comprises the following steps: S1, selecting target microorganisms for expanding culture treatment, and taking bacterial liquid after expanding culture for later use; S2, taking a certain amount of porous ceramic particles, flatly laying the porous ceramic particles on a flat plate, uniformly spraying a certain amount of nutrient solution to the surfaces of the porous ceramic particles, and performing standing for 24 hours for later use; S3, preparing target porous ceramic; S4, preparing a microorganism immobilized product; and S5, preparing the microbial well cementation cement paste. The invention discloses the immobilization method for improving the activity of microorganisms in the well cementation cement paste. The microorganisms are immobilized in a porous material in this way, and then the microorganism well cementation cement paste is prepared. The activity of microorganisms in cement paste can be prolonged, porous ceramic particlesand the well cementation cement paste can be solidified into a cementation body with certain impermeability and mechanical properties through the mineralization effect of the microorganisms, and theservice life of the well cementation cement paste can be greatly prolonged.
本发明属于生物工程与油气开采工程技术领域，具体涉及一种提高固井水泥浆中微生物活性的固载方法，主要包括以下步骤：S1、选取目标微生物进行扩培处理，并取扩培后的菌液备用；S2、取定量多孔陶瓷颗粒平铺在平板上，向其表面均匀喷洒定量的营养液后，静置24小时备用；S3、制备目标多孔陶瓷；S4、制备微生物固载产品；S5、制备微生物固井水泥浆。本发明所述的一种提高固井水泥浆中微生物活性的固载方法，将微生物以此种方式固载于多孔材料中再制备微生物固井水泥浆，可以延长微生物在水泥浆中的活性，通过微生物的成矿作用可以将多孔陶瓷微粒与固井水泥浆固结成具有一定抗渗性和力学性能的胶结体，可以大大延长固井水泥环的服役寿命。
Immobilization method for improving activity of microorganisms in well cementation cement paste
一种提高固井水泥浆中微生物活性的固载方法
LIU TIANLE (Autor:in) / LI LIXIA (Autor:in) / JIANG GUOSHENG (Autor:in) / LIU HAODONG (Autor:in) / ZHENG SHAOJUN (Autor:in) / QIN XIN (Autor:in) / YANG GUOKUN (Autor:in) / YIN SHUTING (Autor:in)
10.07.2020
Patent
Elektronische Ressource
Chinesisch
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