A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Making love and war: jasmonate signaling in herbivore defense and flower development in Nicotiana attenuata
The goal of my work presented in this thesis was to investigate the regulatory role of JAs-homeostasis during the herbivore induced defense activation and floral development in the wild tobacco Nicotiana attenuata. Biosynthetic pathways are most commonly investigated by silencing or overexpression of genes whose products catalyze individual steps within the pathway. However, the complete interruption of a pathway per se prevents the full elucidation of the multiple control mechanisms enabling plants to constantly adjust the output of their signaling pathways. To evade aforementioned limitations, we developed a new approach by ectopically expressing in N. attenuata a gene coding for a methyltransferase that specifically catalyzes the methylation of JA to methyljasmonate (MeJA). Using molecular and chemical analytical methods, I demonstrated the efficiency of this enzymatically redirected JA-flux. The examination of JAs-accumulation patterns upon herbivory in locally treated and untreated systemic leaves revealed tissue-specific consequences of creating a metabolic sink in the JAs-cascade. Furthermore, I show that the metabolic rerouting of JA-flux towards MeJA formation caused no effect on the direct or indirect transcriptional regulation of JA-biosynthesis. ; Zielsetzung meiner Arbeit war die Untersuchung der Rolle der JA-Homöostase für die regulation der induzierbaren Verteidigung sowie der Blütenentwicklung in dem wilden Tabak Nicotiana attenuata. Üblicherweise wird für die Aufschlüsselung eines Biosyntheseweges der Biosyntheseweg selbst mittels Silencing oder Überexpression eines beteiligten Gens unterbrochen. Das verhindert jedoch eine vollständige Entschlüsselung der Selbstregulation des untersuchten Biosynthese- oder Signalweges. Zur Umgehung dieses Problems etablierte und charakterisierte ich eine neue Methode zur Untersuchung des pflanzlichen JA-Signalweges. Ich überexprimierte in N. attenuata ektopisch das Gen für eine Methyltransferase welche JA spezifisch und quantitativ zu Methyljasmonat (MeJA) ...
Making love and war: jasmonate signaling in herbivore defense and flower development in Nicotiana attenuata
The goal of my work presented in this thesis was to investigate the regulatory role of JAs-homeostasis during the herbivore induced defense activation and floral development in the wild tobacco Nicotiana attenuata. Biosynthetic pathways are most commonly investigated by silencing or overexpression of genes whose products catalyze individual steps within the pathway. However, the complete interruption of a pathway per se prevents the full elucidation of the multiple control mechanisms enabling plants to constantly adjust the output of their signaling pathways. To evade aforementioned limitations, we developed a new approach by ectopically expressing in N. attenuata a gene coding for a methyltransferase that specifically catalyzes the methylation of JA to methyljasmonate (MeJA). Using molecular and chemical analytical methods, I demonstrated the efficiency of this enzymatically redirected JA-flux. The examination of JAs-accumulation patterns upon herbivory in locally treated and untreated systemic leaves revealed tissue-specific consequences of creating a metabolic sink in the JAs-cascade. Furthermore, I show that the metabolic rerouting of JA-flux towards MeJA formation caused no effect on the direct or indirect transcriptional regulation of JA-biosynthesis. ; Zielsetzung meiner Arbeit war die Untersuchung der Rolle der JA-Homöostase für die regulation der induzierbaren Verteidigung sowie der Blütenentwicklung in dem wilden Tabak Nicotiana attenuata. Üblicherweise wird für die Aufschlüsselung eines Biosyntheseweges der Biosyntheseweg selbst mittels Silencing oder Überexpression eines beteiligten Gens unterbrochen. Das verhindert jedoch eine vollständige Entschlüsselung der Selbstregulation des untersuchten Biosynthese- oder Signalweges. Zur Umgehung dieses Problems etablierte und charakterisierte ich eine neue Methode zur Untersuchung des pflanzlichen JA-Signalweges. Ich überexprimierte in N. attenuata ektopisch das Gen für eine Methyltransferase welche JA spezifisch und quantitativ zu Methyljasmonat (MeJA) ...
Making love and war: jasmonate signaling in herbivore defense and flower development in Nicotiana attenuata
Stitz, Michael (author) / Baldwin, Ian Thomas / Theißen, Günter / Kuhlemeier, Chris
2014-07-28
Theses
Electronic Resource
English
Nicotiana attenuata protein kinases mediate plant growth and resistance to insect herbivores
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Molecular response of nicotiana attenuata to herbivory and solar UVB: laboratory and fieldwork
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Toxicity of nonylphenol on the cnidarian Hydra attenuata and environmental risk assessment
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