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Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
https://orcid.org/0000-0002-8344-5376
https://orcid.org/0000-0003-3009-1698
AbstractLipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10mM acetic buffer pH5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
HighlightsImmobilization of lipase B from Candida antarctica on metakaolin is achieved.Adsorption of lipase on metakaolin occurs via Langmuir model.The obtained biocatalyst was successfully used in in the synthesis of lipophilic antioxidants (conversion yield of 100%).
Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
https://orcid.org/0000-0002-8344-5376
https://orcid.org/0000-0003-3009-1698
AbstractLipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10mM acetic buffer pH5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
HighlightsImmobilization of lipase B from Candida antarctica on metakaolin is achieved.Adsorption of lipase on metakaolin occurs via Langmuir model.The obtained biocatalyst was successfully used in in the synthesis of lipophilic antioxidants (conversion yield of 100%).
Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
https://orcid.org/0000-0002-8344-5376
https://orcid.org/0000-0003-3009-1698
AbstractLipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10mM acetic buffer pH5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
HighlightsImmobilization of lipase B from Candida antarctica on metakaolin is achieved.Adsorption of lipase on metakaolin occurs via Langmuir model.The obtained biocatalyst was successfully used in in the synthesis of lipophilic antioxidants (conversion yield of 100%).
Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
Jakovetić Tanasković, Sonja (author) / Jokić, Bojan (author) / Grbavčić, Sanja (author) / Drvenica, Ivana (author) / Prlainović, Nevena (author) / Luković, Nevena (author) / Knežević-Jugović, Zorica (author)
Applied Clay Science ; 135 ; 103-111
2016-09-09
9 pages
Article (Journal)
Electronic Resource
English
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