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Optimization method for reinforcing soil body by activating urease-producing microorganisms in situ
The invention discloses an optimization method for reinforcing a soil body by activating urease-producing microorganisms in situ, and belongs to the technical field of microorganism mineralization. The optimization method comprises the following steps: blending a stimulation scheme; blending a carbon source; blending an auxiliary nitrogen source; screening and testing; key screening and concentration testing. According to the optimization method, an initial culture scheme is set firstly, then a suitable optimal carbon source and an optimal auxiliary nitrogen source are screened out through a single-factor experiment, then several remarkable influence factors are further found out through Plackett-Burman experimental design, the reasonable range of all the factors is found out, and the optimal carbon source and the optimal auxiliary nitrogen source are obtained. Finally, the whole stimulation culture scheme is optimized according to a CCD center combination scheme, the biological stimulation effect is improved, good previous conditions are provided for biological mineralization, and then the design of biological stimulation schemes for different soil bodies is effectively achieved.
本发明公开了一种原位激活产脲酶微生物加固土体优化方法，属于微生物矿化技术领域。该优化方法包括以下步骤：刺激方案调配；碳源调配；辅助氮源调配；筛选测试；关键筛选和浓度试验。该优化方法，通过先设置初始培养方案再通过单因素试验筛选出比较适合的最佳碳源和最佳辅助氮源，随后利用Plackett‑Burman实验设计进一步找出其中几种显著影响因子，并找出各种因子的合理范围，最后根据CCD中心组合方案对整个刺激培养方案进行优化，提高生物刺激的效果，为生物矿化提供良好的先前条件，进而实现有效针对不同土体的生物刺激方案的设计。
Optimization method for reinforcing soil body by activating urease-producing microorganisms in situ
The invention discloses an optimization method for reinforcing a soil body by activating urease-producing microorganisms in situ, and belongs to the technical field of microorganism mineralization. The optimization method comprises the following steps: blending a stimulation scheme; blending a carbon source; blending an auxiliary nitrogen source; screening and testing; key screening and concentration testing. According to the optimization method, an initial culture scheme is set firstly, then a suitable optimal carbon source and an optimal auxiliary nitrogen source are screened out through a single-factor experiment, then several remarkable influence factors are further found out through Plackett-Burman experimental design, the reasonable range of all the factors is found out, and the optimal carbon source and the optimal auxiliary nitrogen source are obtained. Finally, the whole stimulation culture scheme is optimized according to a CCD center combination scheme, the biological stimulation effect is improved, good previous conditions are provided for biological mineralization, and then the design of biological stimulation schemes for different soil bodies is effectively achieved.
本发明公开了一种原位激活产脲酶微生物加固土体优化方法，属于微生物矿化技术领域。该优化方法包括以下步骤：刺激方案调配；碳源调配；辅助氮源调配；筛选测试；关键筛选和浓度试验。该优化方法，通过先设置初始培养方案再通过单因素试验筛选出比较适合的最佳碳源和最佳辅助氮源，随后利用Plackett‑Burman实验设计进一步找出其中几种显著影响因子，并找出各种因子的合理范围，最后根据CCD中心组合方案对整个刺激培养方案进行优化，提高生物刺激的效果，为生物矿化提供良好的先前条件，进而实现有效针对不同土体的生物刺激方案的设计。
Optimization method for reinforcing soil body by activating urease-producing microorganisms in situ
一种原位激活产脲酶微生物加固土体优化方法
ZHA FUSHENG (author) / WANG RUNKAI (author) / QIN LIN (author) / KANG BO (author) / WANG QIAO (author)
2024-03-12
Patent
Electronic Resource
Chinese
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