A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
Ud-Daula, Asad (author) / Pfister, Gerd (author) / Schramm, Karl-Werner (author)
Journal of Environmental Science and Health, Part A ; 48 ; 1343-1348
2013-09-19
6 pages
Article (Journal)
Electronic Resource
English
Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
| Online Contents | 2013
ISTA13-catecholamine toxicity and metabolism in the ciliated protozoan, Tetrahymena pyriformis
| Online Contents | 2009
Rapid biotransformation of arsenic by a model protozoan Tetrahymena thermophila
| Online Contents | 2011