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ISTA13‐catecholamine toxicity and metabolism in the ciliated protozoan, Tetrahymena pyriformis
10.1002/tox.20457.abs
A high throughput culture methodology of unicellular eukaryote Tetrahymena pyriformis, strain GL were used for the determination of catecholamines toxicity and their metabolism. Catecholamines exhibited acute toxicity to Tetrahymena cells where dopamine and L‐DOPA showed higher toxic potential of EC10 (0.39 and 0.63 mg/L, respectively) and EC20 (1.1 and 1.0 mg/L, respectively). All the testing catecholamines were highly degradable in the PPY‐medium due to the oxidizing environment during incubation. They were also naturally synthesized and released by Tetrahymena cells into the culture medium and increasingly accumulated with time where as noradrenalin demonstrated significant results. Cells were exposed with physiological concentration (0.12 mg/L) and one higher concentration (8.0 mg/L) of catecholamines, resulting noradrenalin depletion and in vivo generation of a metabolite in response to dopamine with higher concentration treatment. This dopamine metabolite was relatively nonpolar compared with the catecholamines and was eluted later from the reverse phase C‐18 column. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.
ISTA13‐catecholamine toxicity and metabolism in the ciliated protozoan, Tetrahymena pyriformis
10.1002/tox.20457.abs
A high throughput culture methodology of unicellular eukaryote Tetrahymena pyriformis, strain GL were used for the determination of catecholamines toxicity and their metabolism. Catecholamines exhibited acute toxicity to Tetrahymena cells where dopamine and L‐DOPA showed higher toxic potential of EC10 (0.39 and 0.63 mg/L, respectively) and EC20 (1.1 and 1.0 mg/L, respectively). All the testing catecholamines were highly degradable in the PPY‐medium due to the oxidizing environment during incubation. They were also naturally synthesized and released by Tetrahymena cells into the culture medium and increasingly accumulated with time where as noradrenalin demonstrated significant results. Cells were exposed with physiological concentration (0.12 mg/L) and one higher concentration (8.0 mg/L) of catecholamines, resulting noradrenalin depletion and in vivo generation of a metabolite in response to dopamine with higher concentration treatment. This dopamine metabolite was relatively nonpolar compared with the catecholamines and was eluted later from the reverse phase C‐18 column. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.
ISTA13‐catecholamine toxicity and metabolism in the ciliated protozoan, Tetrahymena pyriformis
Ud‐Daula, Asad (author) / Pfister, Gerd (author) / Schramm, Karl‐Werner (author)
Environmental Toxicology ; 24 ; 549-554
2009-12-01
6 pages
Article (Journal)
Electronic Resource
English
ISTA13-catecholamine toxicity and metabolism in the ciliated protozoan, Tetrahymena pyriformis
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