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A platform for research: civil engineering, architecture and urbanism
Nitrogen processing by biofilms along a lowland river continuum
AbstractWhile numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyllaand protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C2H2) block technique and assessing changes in NH, NOxand N2O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NHbefore and after addition of C2H2, occurred mostly in the upper sites with rates up to 1.4 × 10−5 mol m−2 h−1. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10−5 mol m−2 h−1) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NOxby the biofilms following addition of C2H2could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd.
Nitrogen processing by biofilms along a lowland river continuum
AbstractWhile numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyllaand protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C2H2) block technique and assessing changes in NH, NOxand N2O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NHbefore and after addition of C2H2, occurred mostly in the upper sites with rates up to 1.4 × 10−5 mol m−2 h−1. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10−5 mol m−2 h−1) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NOxby the biofilms following addition of C2H2could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd.
Nitrogen processing by biofilms along a lowland river continuum
River Research & Apps
Baldwin, D. S. (author) / Mitchell, A. M. (author) / Rees, G. N. (author) / Watson, G. O. (author) / Williams, J. L. (author)
River Research and Applications ; 22 ; 319-326
2006-03-01
Article (Journal)
Electronic Resource
English
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