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Nitrogen processing by biofilms along a lowland river continuum

Baldwin, D. S. / Mitchell, A. M. / Rees, G. N. / Watson, G. O. / Williams, J. L.

10.1002/rra.896.abs

While numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyll a and protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C₂H₂) block technique and assessing changes in NH $_{4}^{+}$ , NO_x and N₂O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NH $_{4}^{+}$ before and after addition of C₂H₂, occurred mostly in the upper sites with rates up to 1.4 × 10⁻⁵ mol m⁻² h⁻¹. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10⁻⁵ mol m⁻² h⁻¹) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NO_x by the biofilms following addition of C₂H₂ could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd.

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Nitrogen processing by biofilms along a lowland river continuum

Baldwin, D. S. / Mitchell, A. M. / Rees, G. N. / Watson, G. O. / Williams, J. L.

10.1002/rra.896.abs

While numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyll a and protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C₂H₂) block technique and assessing changes in NH $_{4}^{+}$ , NO_x and N₂O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NH $_{4}^{+}$ before and after addition of C₂H₂, occurred mostly in the upper sites with rates up to 1.4 × 10⁻⁵ mol m⁻² h⁻¹. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10⁻⁵ mol m⁻² h⁻¹) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NO_x by the biofilms following addition of C₂H₂ could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd.

Nitrogen processing by biofilms along a lowland river continuum

Baldwin, D. S. / Mitchell, A. M. / Rees, G. N. / Watson, G. O. / Williams, J. L.

10.1002/rra.896.abs

While numerous studies have examined N dynamics along a river continuum, few have specifically examined the role of biofilms. Nitrogen dynamics and microbial community structure were determined on biofilms at six sites along a 120 km stretch of the lowland Ovens River, South Eastern Australia using artificial substrates. Terminal restriction fragment length polymorphism (T‐RFLP), chlorophyll a and protein analyses were used to assess biofilm microbial community composition. N dynamics was determined on the biofilms using the acetylene (C₂H₂) block technique and assessing changes in NH $_{4}^{+}$ , NO_x and N₂O. Unlike microbial community structure, N dynamics were spatially heterogeneous. Nitrification, determined from the difference in accumulation of NH $_{4}^{+}$ before and after addition of C₂H₂, occurred mostly in the upper sites with rates up to 1.4 × 10⁻⁵ mol m⁻² h⁻¹. The highest rates of denitrification occurred in the mid‐reaches of the river (with rates up to 1 × 10⁻⁵ mol m⁻² h⁻¹) but denitrification was not detected in the lower reaches. At the very most, only 50% of the observed uptake of NO_x by the biofilms following addition of C₂H₂ could be accounted for by denitrification. Copyright © 2005 John Wiley & Sons, Ltd.

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Title:

Nitrogen processing by biofilms along a lowland river continuum

Contributors:

Baldwin, D. S. (author) / Mitchell, A. M. (author) / Rees, G. N. (author) / Watson, G. O. (author) / Williams, J. L. (author)

Published in:

River Research and Applications ; 22 ; 319-326

Publication date:

2006-03-01

Size:

8 pages

ISSN:

1535-1467 , 1535-1459

DOI:

https://doi.org/10.1002/rra.896

Type of media:

Article (Journal)

Type of material:

Electronic Resource

Language:

English

Keywords:

denitrification microbial community , T‐RFLP , nitrification , nitrogen

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