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Experimental Investigation and Kinetic Modeling of p‐Nitrophenol and Phenol by Kissiris‐Immobilized Ralstonia eutropha in a Batch Reactor
Co‐metabolic degradation of p‐nitrophenol (PNP) and phenol by kissiris‐immobilized Ralstonia eutropha cells was studied in a batch bioreactor operated in recycling mode (30°C, initial pH of 7.7, aeration rate of 0.5 vvm, and recycling flow rate of 25 mL min−1). Decreasing trends of specific degradation rates of phenol (qSg) and PNP (qSng) with an increase of initial PNP concentration, were mathematically modeled considering the competitive inhibition between growth and non‐growth substrates and self‐inhibition of non‐growth substrate. A non‐linear regression on the equations used in defining of qSg, and qSng, was used to estimate the relevant kinetic parameters. On the best fitted curve, the kinetic parameters were qmax Sg = 0.032 g Phenol g Cell−1 h−1, Km Sg = 442.9 mg L−1, qmax Sng = 0.0056 g Phenol g Cell−1 h−1, Km Sng = 181.2 mg L−1, and KSng = 0.489 mg L−1. These parameters were used for dynamic prediction of the substrates concentration feature during co‐metabolic degradation experiments.
Experimental Investigation and Kinetic Modeling of p‐Nitrophenol and Phenol by Kissiris‐Immobilized Ralstonia eutropha in a Batch Reactor
Co‐metabolic degradation of p‐nitrophenol (PNP) and phenol by kissiris‐immobilized Ralstonia eutropha cells was studied in a batch bioreactor operated in recycling mode (30°C, initial pH of 7.7, aeration rate of 0.5 vvm, and recycling flow rate of 25 mL min−1). Decreasing trends of specific degradation rates of phenol (qSg) and PNP (qSng) with an increase of initial PNP concentration, were mathematically modeled considering the competitive inhibition between growth and non‐growth substrates and self‐inhibition of non‐growth substrate. A non‐linear regression on the equations used in defining of qSg, and qSng, was used to estimate the relevant kinetic parameters. On the best fitted curve, the kinetic parameters were qmax Sg = 0.032 g Phenol g Cell−1 h−1, Km Sg = 442.9 mg L−1, qmax Sng = 0.0056 g Phenol g Cell−1 h−1, Km Sng = 181.2 mg L−1, and KSng = 0.489 mg L−1. These parameters were used for dynamic prediction of the substrates concentration feature during co‐metabolic degradation experiments.
Experimental Investigation and Kinetic Modeling of p‐Nitrophenol and Phenol by Kissiris‐Immobilized Ralstonia eutropha in a Batch Reactor
Motamedi, Mahdi (author) / Habibi, Alireza (author) / Maleki, Mohammad (author) / Vahabzadeh, Farzaneh (author)
CLEAN – Soil, Air, Water ; 43 ; 237-243
2015-02-01
7 pages
Article (Journal)
Electronic Resource
English
Formaldehyde degradation by Ralstonia eutropha in an immobilized cell bioreactor
| Online Contents | 2013
Formaldehyde degradation by Ralstonia eutropha in an immobilized cell bioreactor
| Taylor & Francis Verlag | 2013