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A platform for research: civil engineering, architecture and urbanism
Efficient calcium mineralizing bacterium and application thereof
The invention discloses an efficient calcium mineralizing bacterium and an application thereof, and belongs to the field of microorganisms. The bacterium disclosed by the invention is bacillus cereusEMCa1. The strain is in a short rod shape, and is in a short or long chain, the size of the bacterium is about (1.0-1.2) x (3.0-5.0) [mu]m, the strain is a gram staining positive bacterium, white circular bacterial colonies appear after the strain is cultured for 24 h on an LB agar plate culture medium at 30 DEG C, a diameter is about 5-7 mm, the edge is irregular, and the bacterial colony surfaceis like wax. The induced calcium carbonate deposition characteristic of the strain is as follows: after 20 days of culture, the strain can convert free calcium ions in a coral sand solution into a calcium carbonate precipitate, and in a calcium carbonate deposition experiment, the concentration of calcium ions is reduced from 36.9 mg/L to 7.19 mg/L, and the proportion of calcite in coral sand isincreased from 2% to 20%. According to the efficient calcium mineralizing bacterium and the application thereof, calcium ions can be mineralized to generate calcium carbonate, so that the efficient calcium mineralizing bacterium is applied to the restoration of stone cultural relics.
一株高效钙矿化菌及其应用，属于微生物领域。本发明所述细菌是蜡样芽孢杆菌EMCa1。该菌株为短杆状，成短或长链，菌体大小约为1.0‑1.2×3.0‑5.0μm，革兰氏染色阳性菌，该菌株在LB琼脂平板培养基上30℃培养24小时后，出现白色圆形菌落，直径约5‑7mm，边缘不规则，菌落表面似蜡样，菌株的诱导碳酸钙沉积特性：经过20天的培养，菌株可以将珊瑚砂溶液中的游离钙离子转化为碳酸钙沉淀，碳酸钙沉积实验中，钙离子浓度由36.9mg/L减少到7.19mg/L，珊瑚砂中方解石的比例由2%上升到20%。本发明所述高效钙矿化菌及其应用，能够将钙离子矿化生成碳酸钙，从而应用于石质文物的修复。
Efficient calcium mineralizing bacterium and application thereof
The invention discloses an efficient calcium mineralizing bacterium and an application thereof, and belongs to the field of microorganisms. The bacterium disclosed by the invention is bacillus cereusEMCa1. The strain is in a short rod shape, and is in a short or long chain, the size of the bacterium is about (1.0-1.2) x (3.0-5.0) [mu]m, the strain is a gram staining positive bacterium, white circular bacterial colonies appear after the strain is cultured for 24 h on an LB agar plate culture medium at 30 DEG C, a diameter is about 5-7 mm, the edge is irregular, and the bacterial colony surfaceis like wax. The induced calcium carbonate deposition characteristic of the strain is as follows: after 20 days of culture, the strain can convert free calcium ions in a coral sand solution into a calcium carbonate precipitate, and in a calcium carbonate deposition experiment, the concentration of calcium ions is reduced from 36.9 mg/L to 7.19 mg/L, and the proportion of calcite in coral sand isincreased from 2% to 20%. According to the efficient calcium mineralizing bacterium and the application thereof, calcium ions can be mineralized to generate calcium carbonate, so that the efficient calcium mineralizing bacterium is applied to the restoration of stone cultural relics.
一株高效钙矿化菌及其应用，属于微生物领域。本发明所述细菌是蜡样芽孢杆菌EMCa1。该菌株为短杆状，成短或长链，菌体大小约为1.0‑1.2×3.0‑5.0μm，革兰氏染色阳性菌，该菌株在LB琼脂平板培养基上30℃培养24小时后，出现白色圆形菌落，直径约5‑7mm，边缘不规则，菌落表面似蜡样，菌株的诱导碳酸钙沉积特性：经过20天的培养，菌株可以将珊瑚砂溶液中的游离钙离子转化为碳酸钙沉淀，碳酸钙沉积实验中，钙离子浓度由36.9mg/L减少到7.19mg/L，珊瑚砂中方解石的比例由2%上升到20%。本发明所述高效钙矿化菌及其应用，能够将钙离子矿化生成碳酸钙，从而应用于石质文物的修复。
Efficient calcium mineralizing bacterium and application thereof
一株高效钙矿化菌及其应用
WANG YIMING (author) / YANG XIAOQIAN (author) / LIN XIANGUI (author)
2020-08-28
Patent
Electronic Resource
Chinese
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